Fig. 3

Xylanase activity of the T. aurantiacus strains transformed with a Pgpd::xlnR construct. a The DNS Assay was used to screen 29 transformants grown in Avicel medium for xylanase. b From a subset of the mutants tested in (a), a subset of 4 mutants displaying the highest xylanase activity was used for a shift experiment. The mutants were grown in McClendon’s medium supplemented with soy meal peptone and glucose for 48 h and equal amounts of mycelium were shifted to starvation medium for 72 h and xylanase activity was measured. a Bars represent one biological replicate and the error bars are the standard deviation of 3 technical replicates, the horizontal bars with the asterisk indicate statistical significant difference to the wild-type strains (p value < 0.05), b bars and standard deviation are derived from three biological replicates, the asterisk indicate statistical significant difference to the wild-type strains (p value < 0.05)