Fig. 5
From: Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacus
CRISPR/Cas9 development in T. aurantiacus: a in vitro Cas9 cleavage assay: Agarose gel depicting the uncleaved control fragment and the Cas9 cleavage of the target pyrG sequence with gRNA 1, 2 and 3. b Transformation efficiency per 108 spores with gRNA 1 and 3 containing vectors, selected on hygromycin B uracil plates. Each bar displays the mean and standard deviations from 17 biological replicates. c Deletion efficiencies of both gRNAs targeting Cas9 to the pyrG gene in T. aurantiacus