Table 3 List of protospacers and PAM sequences used in this study
From: Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacus
Target locus | ID | Protospacer sequence (5′–3′) | PAM (5′–3′) |
|---|---|---|---|
pyrG | gRNA 1 | CTTTTGCGCGCGAGCGCCGT | AGG |
pyrG | gRNA 2 | GAGTCTTCCTGCACAGGCCT | GGG |
pyrG | gRNA 3 | TCGGCGCCCGACTTCCCCTA | CGG |