Fig. 5
Changes in the chemical shift (Δδ) observed in the CSP analysis. The CSP analysis of the 13C-labeled lignin oligomer models (50 μM) was performed in the presence of 200 and 350 μM of TrCBM1 in 50 mM acetic acid-d4 buffer prepared using D2O (pD 5.0) and 10% (v/v) DMSO-d6. The Δδ values of signals from the a long- and b short-chain lignin oligomer models in the CSP experiment. The upper panels in a and b show Δδ on the 1H axis. The lower panels show Δδ on the 13C axis. Line broadening of the NMR signals from B 5 in the long-chain lignin models was observed in the presence of 350 μM TrCBM1. Positions that generated extremely low-intensity signals in the absence of TrCBM1 are indicated by asterisks (*). Positions that generated overlapping signals are indicated by # symbols