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Table 2 Comparison of cellulose conversion in EH and SSF experiments at different conditions

From: Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation

Process Total Solid loading (% w/v) D-LA (g/L)# Cellulose to D-LA conversion (%) Cellulose to glucose conversion (%)#
EH 15FPU/50 °C/pH 4.5 10   57.31# 66.64 ± 1.23
EH 15FPU/45 °C/pH 5.5 10   52.13# 60.62 ± 1.43
EH 5FPU/45 °C/pH 5.5 10   43.07# 50.08 ± 1.05
EH 5FPU/37 °C/pH 5.5 10   41.47# 48.22 ± 0.71
Batch SSF (L. bulgaricus WT) 5FPU/37 °C/pH 5.5 5 12.54 ± 0.53 49.11 57.11*
Batch SSF (L. bulgaricus ET45) 15FPU/45 °C/pH 5.5 10 32.37 ± 0.44 63.4 73.72*
Batch SSF (L. bulgaricus ET45) 5FPU/45 °C/pH 5.5 10 30.6 ± 0.61 59.93 69.68*
Batch SSF (L. bulgaricus ET45) 5FPU/45 °C/pH 5.5 5 15.02 ± 0.33 58.83 68.41*
Batch SSF (L. bulgaricus ET45) 3FPU/45 °C/pH 5.5 5 11.82 ± 0.31 46.3 53.84*
Pulse-fed SSF (L. bulgaricus ET45) 5FPU/45 °C/pH 5.5 35 108.58 ± 1.75 60.76 70.65*
  1. #Estimated cellulose to D-LA conversion = Cellulose to glucose conversion*YD-LA/Glucose
  2. *Estimated cellulose to glucose conversion = Cellulose to D-LA conversion/YD-LA/Glucose
  3. YD-LA/Glucose = 0.86, calculated from independent SHF experiments at 45 °C and pH 5.5 (Table 1)
  4. #Measured values are mean ± SD