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Table 2 Substrates/cofactors specificity of YlAraDH1, YlAraDH2, YlMDH1, YlMDH2, YlXDH

From: Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity

Substrates (pHa) YlAraDH1b
(YALI0F02211p)
YlAraDH2b
(YALI0E05643p)
YlMDH1c
(YALI0B16192p)
YlMDH2c
(YALI0D18964p)
YlXDHb
(YALI0E12463p)
Product (activityd) Product (activity) Product (activity) Product (activity) Product (activity)
Mannitol (pH 8.0) Fructose (10.3) Fructose (6.5) Fructose (2.7) Fructose (8.4) Fructose (0.12)
d-Arabitol (pH 8.0) d-Xylulose (5.3) d-Xylulose (6.3) d-Xylulose (0.12) d-Xylulose (0.83) -
Xylitol (pH 8.0) d-Xylulose (3.6) d-Xylulose (4.6) d-Xylulose (0.23) d-Xylulose (1.4) d-Xylulose (4.35)
d-Sorbitol (pH 8.0) l-Sorbose (7.5) l-Sorbose (5.2) l-Sorbose (0.35) l-Sorbose (0.86) Fructose (2.23)
Fructose (pH 6.0) Mannitol (2.6) Mannitol (1.7) Mannitol (0.82) Mannitol (5.6) Mannitol (0.04)
l-Sorbose (pH 6.0) Sorbitol (2.4) Sorbitol (2.6) Sorbitol (0.15) Sorbitol (0.23) -
d-Xylulose (pH 6.0) Arabitol (1.4) Arabitol (2.2) Arabitol (0.12) Arabitol (0.54) Xylitol (2.34)
  1. aReacted at appropriate pH. b,c With NAD(H) or NADP(H) as cofactor separately. d U/mg protein. “-”, undetected activity