Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli W

Table 2 Comparison of diol production (2,3-butanediol and acetoin) in E. coli W Δ4 on different defined media

Experiments	1 AA ± 5 Ace	0.5 AA ± 5 Ace	2 Asn ± 5 Ace	1 Asn ± 5 Ace	1 Asp ± 5 Ace
Total diols [g l⁻¹]	0.62 ± 0.03	0.48 ± 0.02	0.42 ± 0.02	0.35 ± 0.01	0.35 ± 0.01
Diols from medium [g l⁻¹]	0.37 ± 0.01	0.20 ± 0.01	0.19 ± 0.01	0.08 ± 0.01	0.10 ± 0.01
Diols from acetate [g l⁻¹]	0.25 ± 0.04	0.28 ± 0.02	0.24 ± 0.02	0.27 ± 0.01	0.26 ± 0.02
Y [g diols g⁻¹ acetate]	0.053 ± 0.009	0.062 ± 0.005	0.049 ± 0.005	0.052 ± 0.002	0.052 ± 0.004

E. coli W ΔldhA ΔadhE Δpta ΔfrdA 445_Ediss was used for cultivation. Media containing the five amino acid mixture (AA) is compared to medium containing 0.88 g l⁻¹ (onefold) or 1.76 g l⁻¹ (twofold) asparagine (Asn) or aspartate (Asp) and the onefold vitamin concentration. The diol concentration from acetate is calculated by the subtraction of the concentration attributed to the amino acid(s). Mean values and standard deviations were calculated from biological triplicates

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