Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli W

Table 4 Comparison of diol production (2,3-butanediol and acetoin) in E. coli W Δ4 from different TCA intermediates

Compared experiments	1 Mal ± 5 Ace	1 Suc ± 5 Ace	1 OAA ± 5 Ace	1 Asn ± 5 Ace
OD [−]	0.95 ± 0.04	0.99 ± 0.06	0.53 ± 0.03	3.16 ± 0.01
Total diols [g l⁻¹]	0.12 ± 0.01	0.14 ± 0.01	0.02 ± 0.01	0.35 ± 0.01
Diols from medium [g l⁻¹]	0.05 ± 0.01	0.07 ± 0.01	0.03 ± 0.01	0.08 ± 0.01
Diols from acetate [g l⁻¹]	0.06 ± 0.01	0.07 ± 0.02	− 0.01 ± 0.01	0.27 ± 0.01

E. coli W ΔldhA ΔadhE Δpta ΔfrdA 445_Ediss was used for cultivation. Media containing 0.88 g l⁻¹ malate (Mal), succinate (Suc), oxaloacetate (OAA) and asparagine (Asn) with the onefold vitamin concentration were compared. The diol concentration from acetate is calculated by the difference of experiments with and without acetate or by the subtraction of the concentration attributed to the amino acid(s). Mean values and standard deviations were calculated from biological triplicates

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