Fig. 1
Isolation of thermotolerant yeasts from Brazilian ethanol production. Four thermotolerant yeasts have been identified as LBGA-01, LBGA-69, LBGA-157, and LBGA-175. Industrial strain CAT-1 was used as a non-thermotolerant strain control. Cells were grown overnight in YPD medium, and cell concentration was adjusted to 106 cells/ml. Tenfold serial dilutions were spotted in YEPD solid media. Cells were incubated at 30 and 40 °C for 2 days. CAT-1 showed poor growth at 40 °C, and the four strains had the same pattern of growth at 30 and 40 °C