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Table 1 Results of orthogonal experiment

From: Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Treatment

A

B

C

D

E

F

γ-PGA (g/L)

OD600

1

1

1

1

1

1

1

15.39

4.91

2

1

2

2

2

2

2

22.08

3.04

3

1

3

3

3

3

3

27.14

2.15

4

2

1

1

2

2

3

22.51

5.27

5

2

2

2

3

3

1

26.83

5.52

6

2

3

3

1

1

2

25.53

3.61

7

3

1

2

1

3

2

29.86

6.88

8

3

2

3

2

1

3

26.69

7.03

9

3

3

1

3

2

1

22.78

7.38

10

1

1

3

3

2

2

17.69

4.98

11

1

2

1

1

3

3

33.06

3.00

12

1

3

2

2

1

1

21.68

4.42

13

2

1

2

3

1

3

19.09

5.20

14

2

2

3

1

2

1

20.87

4.12

15

2

3

1

2

3

2

29.21

4.45

16

3

1

3

2

3

1

24.31

7.01

17

3

2

1

3

1

2

22.23

7.20

18

3

3

2

1

2

3

35.54

4.43

K1

138.44

128.86

145.19

160.25

130.62

131.87

  

K2

144.04

151.76

155.08

146.48

141.48

146.60

  

K3

161.42

161.88

142.24

135.77

170.41

164.04

  

k1

23.07

21.48

24.20

26.71

21.77

21.98

  

k2

24.01

25.29

25.85

24.41

23.58

24.43

  

k3

26.90

26.98

23.71

22.63

28.40

27.34

  

R

3.83

5.50

2.14

4.08

6.63

5.36