Fig. 2

Optimization of 96-deep well cell culturing and protein expression conditions. a Various media were screened for cell growth by analyzing the log-phase growth rate as a function of glucose (glc) concentration (no glc, blue; 0.1% glc, green; 0.2% glc, red). b SDS-PAGE of SaGGR (MW = 53 kDa) present in soluble protein fractions cultured in ZYP-5052 supplemented with various lactose (lac) and glc levels. Lanes are run in biological duplicate with 0.05% glc and 0.2% lac (1), 0.05% glc and 0.3% lac (2), and 0.05% glc and 0.4% lac (3), 0.1% glc and 0.2% lac (4), 0.2% glc and 0.2% lac (5). These protein expression levels in ZYP-5052 were tested against TB medium induced with 0.05% glc and 0.2% lac (6), with no inducer (7) and induced with 0.1 mM IPTG (8)