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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Fig. 3

Optimization of the protein purification step. a Protein purification of GGR using IMAC tips for an automated 96-well format. Typical protein yields were 38 ± 3 µM GGR when using ≥ 180 mM imidazole. b Due to interference with downstream chemistry, purification was executed by stripping the co-functionalized tips with EDTA at pH 7.4 (dark grey) or pH 5.5 (light grey), where purifications utilizing EDTA at pH 7.4 is comparable to that of using imidazole. c SDS-PAGE showing the relative purity, stability, and yields of SaGGR upon elution (pH 7.4) and after buffer exchanging the eluent to the activity optimum (pH 5.5)

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