Fig. 4
From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography
Comparison among various farnesol extraction and purification techniques following the enzyme activity assay. Liquid–liquid extraction techniques using ethyl acetate (EtOAc) or cold brine-saturated ethyl acetate (EtOAc/NaCl) were far superior for farnesol recovery in an automated 96-well format than solid-phase C18 methods utilizing methanol (MeOH) or 50% MeOH in EtOAc (MeOH-50)