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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Fig. 5

Optimization of acoustic droplet ejection and application of acoustic printing for the time-course assay. a Nanoliters of coomassie stain transferred by acoustic droplet ejection to a silica-TLC plate. The optimal volume at 50 nL, circled, was determined as the best volume for a simultaneous 48-well TLC analysis. b A time-course assay of 30 µM WT SaGGR incubated with 2 mM farnesol quenched with epoxidation reagents every minute and spotted in a separate lane. As the assay evolves to its 48-min endpoint, the bands corresponding to the epoxide derivatives of H0-, H2- and H4-FOH are indicated by Rf (1), Rf (2) and Rf (3), respectively, and increase in intensity while side-product bands Rf (s2) and Rf (s3) remain relatively constant with time. Quantitative Rf values for all bands are listed in Additional file 1: Table S1. c Gas chromatograms derived from the time-course described in b depict farnesol (H0), di- (H2) and tetrahydrofarnesols (H4)

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