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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Fig. 7

Comparison of select mutants within the L377X library. a Normalized GC–MS traces of products isolated after 80 min of incubation time with 30 µM WT SaGGR (grey), L377M (green), L377D (magenta), L377G (orange), L377N (teal), L377R (blue), or L377T (red) with 500 µM farnesol at pH 5.5 and 37 °C. b Time-course comparing product formation among all mutants at 0, 20, 50 and 80 min. c Relative turnover numbers for all mutants. The color schemes used in the bottom panels are consistent with that used in the top panel

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