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Fig. 8 | Biotechnology for Biofuels

Fig. 8

From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Fig. 8

View of the active site from the entry point of the substrate-binding tunnel. Leu377 from the substrate-binding domain and Val89 from the catalytic domain in WT SaGGR form van der Waals interactions between methylene groups (grey) that comprise the tunnel’s native contacts. This tunnel appears to be occluded in the L377M mutant, which is the only mutant shown to decrease reductase activity toward farnesol. In the remaining mutants enhancing reductase activity, they either expand the substrate-binding tunnel (L377G or L377T) or provide charges that could enhance protein–solvent interactions via hydrogen-bonding oxygens (red) or nitrogens (blue) (L377N, L377R, L377D, L377H)

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