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Table 1 Overview of selected mutants from the L377X library

From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Well Codon Substitution Amino Acid Substitution Specific Activity, (nmol Prenyl Groups Reduced h−1)
A2 CTG WT 3.6 ± 0.7
B2 ACG L377T 9 ± 1
C7 AAT L377N 7.2 ± 0.5
C10 GGG L377G 7.8 ± 0.9
E1 ATG L377M 0.8 ± 0.2
E9 GAT L377D 8.6 ± 0.6
G11 CGT L377R 11 ± 1