Table 1 Overview of selected mutants from the L377X library
From: An automated workflow to screen alkene reductases using high-throughput thin layer chromatography
Well | Codon Substitution | Amino Acid Substitution | Specific Activity, (nmol Prenyl Groups Reduced h−1) |
|---|---|---|---|
A2 | CTG | WT | 3.6 ± 0.7 |
B2 | ACG | L377T | 9 ± 1 |
C7 | AAT | L377N | 7.2 ± 0.5 |
C10 | GGG | L377G | 7.8 ± 0.9 |
E1 | ATG | L377M | 0.8 ± 0.2 |
E9 | GAT | L377D | 8.6 ± 0.6 |
G11 | CGT | L377R | 11 ± 1 |