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Table 4 Strains and plasmids used in this study

From: ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production

  Description Reference
Strain
7D S. cerevisiae CEN.PK113-7D. MATa; URA3; TRP1; LEU2; HIS3; MAL2-8c; SUC2 Euroscarf
7D-atp2 7D with genomic integration of the T. reesei ATPase β subunit gene atp2 (pTEF1-atp2-tCYC1) at X2 locus This study
7D-atpAGD (2A) 7D with genomic integration of the E. coli ATPase α, β and γ subunit genes atpAGD joined via viral 2A peptides at X2 locus This study
5D S. cerevisiae CEN.PK113-5D. MATa; ura3-52; TRP1; LEU2; HIS3; MAL2-8c; SUC2 Euroscarf
5D (E.V.) 5D transformed with the empty vector pRSII326 This study
5D-atp2 5D transformed with pRSII326-atp2 This study
5D-atpAGD 5D transformed with pRSII326-atpAGD This study
5D-atpAGD (2A) 5D transformed with pRSII326-atpAGD (2A) This study
Plasmids
pCfB2312 Cas9 Expression Vector [35]
pCfB2899 EasyClone-MarkerFree Integrative Vector for insertion into Chromosome X: 194944...195980 (X2 locus) [35]
pCfB3020 EasyClone-MarkerFree gRNA Helper Vector for insertion into X2 locus [35]
pCfB2899-atp2 pCfB2899 carrying the T. reesei ATPase β subunit gene atp2 (pTEF1-atp2-tCYC1) This study
pCfB2899-atpAGD (2A) pCfB2899 carrying the E. coli ATPase α, β and γ subunit genes atpAGD joined with viral 2A peptide sequences under the control of TEF1 promoter This study
pRSII326 Shuttle vector with URA3 marker and 2µ ORI-STB. Addgene ID: 35469 [36]
pRSII326-atp2 pRSII326 with T. reesei ATPase β subunit gene atp2 (pTEF1-atp2-tCYC1) This study
pRSII326-atpAGD pRSII326 with E. coli ATPase α subunit gene atpA (pTPI-atpA-tCPS1), subunit β gene atpD (pTEF1-atpD-tPRM9) and γ subunit gene atpG (pGPM1-atpG-tHIS5) This study
pRSII326-atpAGD (2A) pRSII326 with E. coli ATPase α, β and γ subunit genes atpAGD joined with viral 2A peptide sequences under the control of TEF1 promoter This study
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