| Description | Reference |
---|---|---|
Strain | ||
7D | S. cerevisiae CEN.PK113-7D. MATa; URA3; TRP1; LEU2; HIS3; MAL2-8c; SUC2 | Euroscarf |
7D-atp2 | 7D with genomic integration of the T. reesei ATPase β subunit gene atp2 (pTEF1-atp2-tCYC1) at X2 locus | This study |
7D-atpAGD (2A) | 7D with genomic integration of the E. coli ATPase α, β and γ subunit genes atpAGD joined via viral 2A peptides at X2 locus | This study |
5D | S. cerevisiae CEN.PK113-5D. MATa; ura3-52; TRP1; LEU2; HIS3; MAL2-8c; SUC2 | Euroscarf |
5D (E.V.) | 5D transformed with the empty vector pRSII326 | This study |
5D-atp2 | 5D transformed with pRSII326-atp2 | This study |
5D-atpAGD | 5D transformed with pRSII326-atpAGD | This study |
5D-atpAGD (2A) | 5D transformed with pRSII326-atpAGD (2A) | This study |
Plasmids | ||
pCfB2312 | Cas9 Expression Vector | [35] |
pCfB2899 | EasyClone-MarkerFree Integrative Vector for insertion into Chromosome X: 194944...195980 (X2 locus) | [35] |
pCfB3020 | EasyClone-MarkerFree gRNA Helper Vector for insertion into X2 locus | [35] |
pCfB2899-atp2 | pCfB2899 carrying the T. reesei ATPase β subunit gene atp2 (pTEF1-atp2-tCYC1) | This study |
pCfB2899-atpAGD (2A) | pCfB2899 carrying the E. coli ATPase α, β and γ subunit genes atpAGD joined with viral 2A peptide sequences under the control of TEF1 promoter | This study |
pRSII326 | Shuttle vector with URA3 marker and 2µ ORI-STB. Addgene ID: 35469 | [36] |
pRSII326-atp2 | pRSII326 with T. reesei ATPase β subunit gene atp2 (pTEF1-atp2-tCYC1) | This study |
pRSII326-atpAGD | pRSII326 with E. coli ATPase α subunit gene atpA (pTPI-atpA-tCPS1), subunit β gene atpD (pTEF1-atpD-tPRM9) and γ subunit gene atpG (pGPM1-atpG-tHIS5) | This study |
pRSII326-atpAGD (2A) | pRSII326 with E. coli ATPase α, β and γ subunit genes atpAGD joined with viral 2A peptide sequences under the control of TEF1 promoter | This study |