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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Rapid optimisation of cellulolytic enzymes ratios in Saccharomyces cerevisiae using in vitro SCRaMbLE

Fig. 2

The relationship between Cel5A and Cel3A activity compared to BPNPG5 activity in S. cerevisiae strains containing in vitro SCRaMBLEd plasmids. After 48 h of cultivation, the culture supernatant of 160 strains containing in vitro SCRaMbLEd plasmids was used for enzyme assays; Cel3A activity was measured on pNPG (y-axis), Cel5A activity was measured using a DNS assay on CMC (x-axis) and the synergistic activity of both enzymes was measured on BPNPG5 substrate (diameter of spheres). To highlight 12 strains with the highest synergistic enzyme activity, spheres were coloured green

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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