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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Rapid optimisation of cellulolytic enzymes ratios in Saccharomyces cerevisiae using in vitro SCRaMbLE

Fig. 4

Supernatant enzyme activity on BPNPG5 using mixtures of Cel3A and Cel5A at different ratios. Enzyme mixtures were prepared using the culture supernatants of two S. cerevisiae strains producing Cel3A and Cel5A. Activity (U) of Cel3A and Cel5A was measured using p-NPG and DNS assays, respectively, and mixtures of each were prepared using various ratios of Cel3A and Cel5A activity units from 0 to 1 in increments of 0.1 while the total number of activity units was 100 in all samples

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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