Fig. 5

Average copy number of CEL3A, CEL5A and hphMX4 in populations of S. cerevisiae containing in vitro SCRaMbLEd plasmids. Strains H1–6 (high BPNPG5 activity group) exhibited the highest enzyme activity and strains I1–6 (intermediate BPNPG5 activity group) exhibited approximately the median enzyme activities. The box represents the 25th to 75th percentiles and the line across the box represents the median. The copy number per cell of CEL3A and CEL5A was significantly greater in strains H1–H6 (p < 0.02, p < 0.05, respectively) while there was no difference in the copy number of the hphMX4 marker (p > 0.05), suggesting that plasmid copy number was consistent between groups. All gene copy numbers are reported per cell by comparison to a reference gene (TAF10) which occurs as a single copy in the haploid genome