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Table 1 Cell mass yields (Yx/s [g/g]), lipid yields (Yp/s [g/g]), consumption (Qs [g/L × h]) and production rates (Qp [g/L × h]) in different cultivation periods in direct comparison between pulsed standard and adjusted pulsed process strategies

From: Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Cultivation phase [h] Yx/s [g/g] Yp/s [g/g] Qs [g/L × h] Qp [g/L × h]
c(Glu) 90 g/L CFS Glu c(Xyl) 60 g/L CFS Xyl c(Glu) 90 g/L CFS Glu c(Xyl) 60 g/L CFS Xyl c(Glu) 90 g/L CFS Glu c(Xyl) 60 g/L CFS Xyl c(Glu) 90 g/L CFS Glu c(Xyl) 60 g/L CFS Xyl
0–24 0.34 ± 0.06 0.33 ± 0.11 0.56 ± 0.00* 0.39 ± 0.00* 0.04 ± 0.01 0.04 ± 0.00 0.05 ± 0.01 0.04 ± 0.01 0.57 ± 0.02 0.53 ± 0.22 0.39 ± 0.07 0.24 ± 0.04 0.02 ± 0.01 0.02 ± 0.01 0.02 ± 0.01 0.01 ± 0.00
24–48 0.26 ± 0.02 0.23 ± 0.09 0.32 ± 0.02 0.28 ± 0.01 0.04 ± 0.00 0.04 ± 0.03 0.04 ± 0.01 0.03 ± 0.00 1.33 ± 0.12 1.79 ± 0.25 1.37 ± 0.14 1.26 ± 0.08 0.05 ± 0.00 0.06 ± 0.04 0.05 ± 0.01 0.04 ± 0.01
48–72 0.22 ± 0.04 0.31 ± 0.02 0.31 ± 0.03 0.24 ± 0.00 0.08 ± 0.02 0.09 ± 0.03 0.10 ± 0.02 0.06 ± 0.01 1.00 ± 0.01 0.99 ± 0.06 1.06 ± 0.13 1.13 ± 0.02 0.07 ± 0.02 0.08 ± 0.02 0.09 ± 0.01 0.06 ± 0.01
72–96 0.23 ± 0.01 0.20 ± 0.04 0.26 ± 0.00* 0.22 ± 0.01* 0.10 ± 0.03 0.10 ± 0.02 0.06 ± 0.05 0.08 ± 0.00 0.63 ± 0.04 0.79 ± 0.09 0.91 ± 0.15 0.85 ± 0.06 0.06 ± 0.02 0.07 ± 0.01 0.05 ± 0.05 0.07 ± 0.01
96–120 0.13 ± 0.01 0.15 ± 0.09 0.37 ± 0.03* 0.17 ± 0.02* 0.04 ± 0.00* 0.10 ± 0.00* 0.11 ± 0.01 0.06 ± 0.02 0.58 ± 0.12 0.63 ± 0.04 0.55 ± 0.01 0.68 ± 0.05 0.02 ± 0.00* 0.06 ± 0.01* 0.06 ± 0.00 0.04 ± 0.01
120–144 0.21 ± 0.02 0.22 ± 0.04 0.19 ± 0.05 0.19 ± 0.01 0.05 ± 0.06 0.09 ± 0.02 0.10 ± 0.06 0.03 ± 0.01 0.56 ± 0.05 0.58 ± 0.13 0.43 ± 0.00 0.62 ± 0.15 0.02 ± 0.03 0.05 ± 0.02 0.05 ± 0.03 0.02 ± 0.01
N-metabolization phase 0.25 ± 0.02 0.27 ± 0.07 0.34 ± 0.02 0.28 ± 0.01 0.05 ± 0.01 0.05 ± 0.03 0.06 ± 0.01 0.04 ± 0.00 0.91 ± 0.03 1.04 ± 0.03 0.89 ± 0.06 0.83 ± 0.04 0.05 ± 0.01 0.05 ± 0.02 0.06 ± 0.01 0.04 ± 0.01
N-limitation phase 0.07 ± 0.02 0.12 ± 0.02 0.15 ± 0.01 0.14 ± 0.02 0.06 ± 0.00* 0.10 ± 0.00* 0.09 ± 0.04 0.06 ± 0.01 0.58 ± 0.00 0.61 ± 0.08 0.58 ± 0.04 0.66 ± 0.05 0.04 ± 0.00* 0.06 ± 0.01* 0.05 ± 0.03 0.04 ± 0.00
Total 0.19 ± 0.01 0.22 ± 0.05 0.26 ± 0.01* 0.22 ± 0.00* 0.06 ± 0.00 0.07 ± 0.02 0.07 ± 0.01 0.05 ± 0.01 0.74 ± 0.01 0.83 ± 0.03 0.73 ± 0.05 0.74 ± 0.00 0.04 ± 0.00 0.06 ± 0.02 0.05 ± 0.01 0.04 ± 0.00
  1. Yx/s = cell mass specific yield; Yp/s = product specific yield; Qs = volumetric consumption rate; Qp = volumetric production rate; c(Glu) 90 g/L = standard process with daily glucose restock to 90 g/L; CFS Glu = calculated feed strategy for glucose; c(Xyl) 60 g/L = standard process with daily xylose restock to 60 g/L; CFS Xyl = calculated feed strategy for xylose
  2. * Indicates statistical significant differences (p = 0.05)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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