Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte, Olga; Kugel, Michaela; Ochsenreither, Katrin

doi:10.1186/s13068-020-01824-7

Biotechnology for Biofuels and Bioproducts

Table 1 Cell mass yields (Y_x/s [g/g]), lipid yields (Y_p/s [g/g]), consumption (Q_s [g/L × h]) and production rates (Q_p [g/L × h]) in different cultivation periods in direct comparison between pulsed standard and adjusted pulsed process strategies

From: Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Cultivation phase [h]	Y_x/s [g/g]				Y_p/s [g/g]				Q_s [g/L × h]				Q_p [g/L × h]
Cultivation phase [h]	c(Glu) 90 g/L	CFS Glu	c(Xyl) 60 g/L	CFS Xyl	c(Glu) 90 g/L	CFS Glu	c(Xyl) 60 g/L	CFS Xyl	c(Glu) 90 g/L	CFS Glu	c(Xyl) 60 g/L	CFS Xyl	c(Glu) 90 g/L	CFS Glu	c(Xyl) 60 g/L	CFS Xyl
0–24	0.34 ± 0.06	0.33 ± 0.11	0.56 ± 0.00*	0.39 ± 0.00*	0.04 ± 0.01	0.04 ± 0.00	0.05 ± 0.01	0.04 ± 0.01	0.57 ± 0.02	0.53 ± 0.22	0.39 ± 0.07	0.24 ± 0.04	0.02 ± 0.01	0.02 ± 0.01	0.02 ± 0.01	0.01 ± 0.00
24–48	0.26 ± 0.02	0.23 ± 0.09	0.32 ± 0.02	0.28 ± 0.01	0.04 ± 0.00	0.04 ± 0.03	0.04 ± 0.01	0.03 ± 0.00	1.33 ± 0.12	1.79 ± 0.25	1.37 ± 0.14	1.26 ± 0.08	0.05 ± 0.00	0.06 ± 0.04	0.05 ± 0.01	0.04 ± 0.01
48–72	0.22 ± 0.04	0.31 ± 0.02	0.31 ± 0.03	0.24 ± 0.00	0.08 ± 0.02	0.09 ± 0.03	0.10 ± 0.02	0.06 ± 0.01	1.00 ± 0.01	0.99 ± 0.06	1.06 ± 0.13	1.13 ± 0.02	0.07 ± 0.02	0.08 ± 0.02	0.09 ± 0.01	0.06 ± 0.01
72–96	0.23 ± 0.01	0.20 ± 0.04	0.26 ± 0.00*	0.22 ± 0.01*	0.10 ± 0.03	0.10 ± 0.02	0.06 ± 0.05	0.08 ± 0.00	0.63 ± 0.04	0.79 ± 0.09	0.91 ± 0.15	0.85 ± 0.06	0.06 ± 0.02	0.07 ± 0.01	0.05 ± 0.05	0.07 ± 0.01
96–120	0.13 ± 0.01	0.15 ± 0.09	0.37 ± 0.03*	0.17 ± 0.02*	0.04 ± 0.00*	0.10 ± 0.00*	0.11 ± 0.01	0.06 ± 0.02	0.58 ± 0.12	0.63 ± 0.04	0.55 ± 0.01	0.68 ± 0.05	0.02 ± 0.00*	0.06 ± 0.01*	0.06 ± 0.00	0.04 ± 0.01
120–144	0.21 ± 0.02	0.22 ± 0.04	0.19 ± 0.05	0.19 ± 0.01	0.05 ± 0.06	0.09 ± 0.02	0.10 ± 0.06	0.03 ± 0.01	0.56 ± 0.05	0.58 ± 0.13	0.43 ± 0.00	0.62 ± 0.15	0.02 ± 0.03	0.05 ± 0.02	0.05 ± 0.03	0.02 ± 0.01
N-metabolization phase	0.25 ± 0.02	0.27 ± 0.07	0.34 ± 0.02	0.28 ± 0.01	0.05 ± 0.01	0.05 ± 0.03	0.06 ± 0.01	0.04 ± 0.00	0.91 ± 0.03	1.04 ± 0.03	0.89 ± 0.06	0.83 ± 0.04	0.05 ± 0.01	0.05 ± 0.02	0.06 ± 0.01	0.04 ± 0.01
N-limitation phase	0.07 ± 0.02	0.12 ± 0.02	0.15 ± 0.01	0.14 ± 0.02	0.06 ± 0.00*	0.10 ± 0.00*	0.09 ± 0.04	0.06 ± 0.01	0.58 ± 0.00	0.61 ± 0.08	0.58 ± 0.04	0.66 ± 0.05	0.04 ± 0.00*	0.06 ± 0.01*	0.05 ± 0.03	0.04 ± 0.00
Total	0.19 ± 0.01	0.22 ± 0.05	0.26 ± 0.01*	0.22 ± 0.00*	0.06 ± 0.00	0.07 ± 0.02	0.07 ± 0.01	0.05 ± 0.01	0.74 ± 0.01	0.83 ± 0.03	0.73 ± 0.05	0.74 ± 0.00	0.04 ± 0.00	0.06 ± 0.02	0.05 ± 0.01	0.04 ± 0.00

Y_x/s = cell mass specific yield; Y_p/s = product specific yield; Q_s = volumetric consumption rate; Q_p = volumetric production rate; c(Glu) 90 g/L = standard process with daily glucose restock to 90 g/L; CFS Glu = calculated feed strategy for glucose; c(Xyl) 60 g/L = standard process with daily xylose restock to 60 g/L; CFS Xyl = calculated feed strategy for xylose
* Indicates statistical significant differences (p = 0.05)

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