|
Feeding strategy
|
Daily manual restock
|
Batch/continuous fed-batch
|
Daily manual restock
|
Batch/continuous fed-batch
|
|
Feeding status
|
Un-optimized
|
Optimized
|
Un-optimized
|
Optimized
|
|
Total sugar amount applied [g]
|
269
|
160
|
204
|
150
|
|
Total sugar amount metabolized [g]
|
150.66 ± 11.71
|
145.35 ± 6.05
|
147.88 ± 1.17
|
138.46 ± 2.40
|
|
% sugar used by yeasts
|
56.01 ± 4.35
|
90.84 ± 3.78
|
72.49 ± 0.56
|
92.30 ± 1.61
|
|
Final CDW [g/L]
|
19.38 ± 2.37
|
26.78 ± 3.5
|
27.98 ± 2.65
|
25.38 ± 0.23
|
|
Final lipid content [% FAME/CDW]
|
30.82 ± 1.84
|
28.34 ± 2.38
|
26.87 ± 2.04
|
27.53 ± 1.07
|
|
Final lipid titer [g/L]
|
5.98 ± 0.40
|
7.41 ± 1.55
|
7.54 ± 1.28
|
6.68 ± 0.43
|
|
Final organic acid concentration [g/L]
|
GA: 19.78 ± 0.98
|
GA: 10.38 ± 3.57
|
XA: 0.90 ± 0.16
|
XA: 0.64 ± 0.11
|
|
Total consumption rate [g/(L × h)]
|
0.71 ± 0.06
|
0.88 ± 0.06
|
0.87 ± 0.03
|
0.78 ± 0.01
|
|
Total cell mass production rate [g/(L × h)]
|
0.14 ± 0.02
|
0.19 ± 0.02
|
0.20 ± 0.02
|
0.18 ± 0.02
|
|
Total lipid production rate [g/(L × h)]
|
0.04 ± 0.00
|
0.05 ± 0.01
|
0.05 ± 0.01
|
0.05 ± 0.00
|
|
Total organic acid production rate [g/(L × h)]
|
GA: 0.14 ± 0.01
|
GA: 0.07 ± 0.03
|
XA: 0.006 ± 0.001
|
XA: 0.0004 ± 0.0001
|
|
Total Yx/s [g/g]
|
0.19 ± 0.01
|
0.21 ± 0.01
|
0.26 ± 0.01
|
0.22 ± 0.00
|
|
Total Yl/s [g/g]
|
0.06 ± 0.00
|
0.06 ± 0.01
|
0.07 ± 0.01
|
0.06 ± 0.00
|
|
Total produced CO2 [g]
|
84.62
|
109.79
|
84.96
|
118.90
|
|
Total produced CO2 per CDW [g/g]
|
2.98 ± 0.36
|
3.58 ± 0.38
|
2.42 ± 0.05
|
3.83 ± 0.04
|
