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Table 2 Comparison of high sugar standard cultivation and reduced sugar cultivation with continuous feed

From: Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

  c(Glu) 90 g/L Cont. CFS Glu c(Xyl) 60 g/L Cont. CFS Xyl
Feeding strategy Daily manual restock Batch/continuous fed-batch Daily manual restock Batch/continuous fed-batch
Feeding status Un-optimized Optimized Un-optimized Optimized
Total sugar amount applied [g] 269 160 204 150
Total sugar amount metabolized [g] 150.66 ± 11.71 145.35 ± 6.05 147.88 ± 1.17 138.46 ± 2.40
% sugar used by yeasts 56.01 ± 4.35 90.84 ± 3.78 72.49 ± 0.56 92.30 ± 1.61
Final CDW [g/L] 19.38 ± 2.37 26.78 ± 3.5 27.98 ± 2.65 25.38 ± 0.23
Final lipid content [% FAME/CDW] 30.82 ± 1.84 28.34 ± 2.38 26.87 ± 2.04 27.53 ± 1.07
Final lipid titer [g/L] 5.98 ± 0.40 7.41 ± 1.55 7.54 ± 1.28 6.68 ± 0.43
Final organic acid concentration [g/L] GA: 19.78 ± 0.98 GA: 10.38 ± 3.57 XA: 0.90 ± 0.16 XA: 0.64 ± 0.11
Total consumption rate [g/(L × h)] 0.71 ± 0.06 0.88 ± 0.06 0.87 ± 0.03 0.78 ± 0.01
Total cell mass production rate [g/(L × h)] 0.14 ± 0.02 0.19 ± 0.02 0.20 ± 0.02 0.18 ± 0.02
Total lipid production rate [g/(L × h)] 0.04 ± 0.00 0.05 ± 0.01 0.05 ± 0.01 0.05 ± 0.00
Total organic acid production rate [g/(L × h)] GA: 0.14 ± 0.01 GA: 0.07 ± 0.03 XA: 0.006 ± 0.001 XA: 0.0004 ± 0.0001
Total Yx/s [g/g] 0.19 ± 0.01 0.21 ± 0.01 0.26 ± 0.01 0.22 ± 0.00
Total Yl/s [g/g] 0.06 ± 0.00 0.06 ± 0.01 0.07 ± 0.01 0.06 ± 0.00
Total produced CO2 [g] 84.62 109.79 84.96 118.90
Total produced CO2 per CDW [g/g] 2.98 ± 0.36 3.58 ± 0.38 2.42 ± 0.05 3.83 ± 0.04
  1. c(Glu) 90 g/L = standard process with daily glucose restock to 90 g/L; Cont. CFS Glu = calculated feed strategy for glucose with continuous feed; c(Xyl) 60 g/L =  standard process with daily xylose restock to 60 g/L; Cont. CFS Xyl = calculated feed strategy for xylose with continuous feed, Yx/s = cell mass specific yield; Yl/s = lipid specific yield, CDW = cell dry weight
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