Fig. 3From: Evolutionary engineering of E. coli MG1655 for tolerance against isoprenolReconstitution of yghB mutation. a Deletion upstream of yghB and annotation of putative regulator motif. b Complementation of yghB-knock-out mutation with a plasmid carrying yghB under control of an inducible promotor (IPTG) under isoprenol stress (50Â mM). Relative fitness is defined as the observed growth rate divided by the growth rate of the initial MG1655 (WT) under identical conditions. Values represent the mean value of three biological replicates. Error bars indicate standard deviationBack to article page