Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol

Table 2 Mean transcriptional response of adapted strains T7A-C against Isoprenol stress compared to the parental strain MG1655

Transcript name	Cuffdiff	DESeq	edgeR	Description
ompF	3.66	5.52*	5.76*	Outer membrane protein F (Porin)
alaE	5.20*	3.60	5.24	Ala-Ala exporter
yghB	3.87*	3.66	3.84*	Membrane protein
ilvX	2.18	3.07*	3.77*	Unknown
ilvG, ilvM	2.41*	2.26*	2.72*	Val and Ile synthesis
ldrA	0.00	1.19	4.94*	Peptide toxin
glxK	1.93*	1.11	1.62	Glycerate kinase II
yahO	1.69*	1.35	1.55*	Tolerance against X and UV radiation
yhaH	1.59*	1.30	1.55*	Inner membrane protein
yodC	1.68*	0.78	1.40	Fimbrial tip-adhesin
ynfQ	− 3.06*	− 1.86	− 3.25*	Small protein cold shock-induced
citC	− 2.77	− 2.72	− 2.97*	Citrate lyase synthetase
glgS	− 3.20*	− 2.86	− 3.09*	Regulates motility and biofilm
flu	− 3.29*	− 3.07	− 3.25*	Small RNA, membrane protein, aggregation
cspI	− 3.64*	− 2.60	− 3.51*	Cold-shock protein
isrC	− 3.59*	− 3.17*	− 3.49*	Small RNA
menA	NaN	− 6.19*	− 11.32*	Membrane protein
hslU	NaN	− 8.74	− 14.37*	ATPase component of HslVU protease
sapB	− 13.57	− 7.54	− 15.13*	Membrane subunit of putative putrescine exporter
rraA	− 13.13	− 9.27*	− 14.45*	Inhibits ribonuclease activity

Log2 fold-changes were determined using cuffdiff, DESeq, and edgeR, values are mean fold-change of strain T7A, T7B, and T7C compared to the parental strain. Asterisks indicate statistically significant differential regulation (p < 0.05) for all three adapted strains. Protein functions are taken from ecocyc.org

ISSN: 2731-3654