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Table 2 Mean transcriptional response of adapted strains T7A-C against Isoprenol stress compared to the parental strain MG1655

From: Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol

Transcript name Cuffdiff DESeq edgeR Description
ompF 3.66 5.52* 5.76* Outer membrane protein F (Porin)
alaE 5.20* 3.60 5.24 Ala-Ala exporter
yghB 3.87* 3.66 3.84* Membrane protein
ilvX 2.18 3.07* 3.77* Unknown
ilvG, ilvM 2.41* 2.26* 2.72* Val and Ile synthesis
ldrA 0.00 1.19 4.94* Peptide toxin
glxK 1.93* 1.11 1.62 Glycerate kinase II
yahO 1.69* 1.35 1.55* Tolerance against X and UV radiation
yhaH 1.59* 1.30 1.55* Inner membrane protein
yodC 1.68* 0.78 1.40 Fimbrial tip-adhesin
ynfQ − 3.06* − 1.86 − 3.25* Small protein cold shock-induced
citC − 2.77 − 2.72 − 2.97* Citrate lyase synthetase
glgS − 3.20* − 2.86 − 3.09* Regulates motility and biofilm
flu − 3.29* − 3.07 − 3.25* Small RNA, membrane protein, aggregation
cspI − 3.64* − 2.60 − 3.51* Cold-shock protein
isrC − 3.59* − 3.17* − 3.49* Small RNA
menA NaN − 6.19* − 11.32* Membrane protein
hslU NaN − 8.74 − 14.37* ATPase component of HslVU protease
sapB − 13.57 − 7.54 − 15.13* Membrane subunit of putative putrescine exporter
rraA − 13.13 − 9.27* − 14.45* Inhibits ribonuclease activity
  1. Log2 fold-changes were determined using cuffdiff, DESeq, and edgeR, values are mean fold-change of strain T7A, T7B, and T7C compared to the parental strain. Asterisks indicate statistically significant differential regulation (p < 0.05) for all three adapted strains. Protein functions are taken from ecocyc.org