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Table 3 Background strains

From: Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol

Strain

Genotype

References

Escherichia coli MG1655

K-12 F– λ– ilvG– rfb-50 rph-1

[40]

Escherichia coli DH5α

F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ–

–

Escherichia coli BW25113

rrnB3 DElacZ4787 hsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1

[42]

Keio ΔmarC

BW25113 marC::kanR

[42]

Keio Δrob

BW25113 rob::kanR

[42]

Keio ΔyghB

BW25113 yghB::kanR

[42]