Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol

Table 4 Strains constructed in this work

No.	Strain genotype	Resistance	Alias
557	E. coli MG1655 Ptrc10 yghB	ampR cmR	yghB
564	E. coli MG1655 Ptrc10 empty	ampR cmR	Empty vector
952	E. coli MG1655 ΔyghB781::kanR	kanR	ΔyghB
953	E. coli MG1655 ΔmarC750::kanR	kanR	ΔmarC
954	E. coli MG1655 Δrob-721::kanR	kanR	Δrob
960	E. coli MG1655 ΔmarC750::kanR Ptrc10 marC35 kanR	kanR ampR cmR	ΔmarC marC35
961	E. coli MG1655 Δrob-721::kanR Ptrc10 robH	ampR cmR kanR	Δrob robH
1084	E. coli MG1655 ΔyghB781::kanR Ptrc10 yghB	ampR cmR kanR	ΔyghB yghB
1085	E. coli MG1655 ΔyghB781::kanR Ptrc10 empty	ampR cmR kanR	ΔyghB empty vector
1086	E. coli MG1655 Δrob-721::kanR Ptrc10 empty	ampR cmR kanR	Δrob empty vector
1087	E. coli MG1655 ΔmarC750::kanR Ptrc10 empty	ampR cmR kanR	ΔmarC empty vector

Alias is used throughout the manuscript. The reference number refers to the internal strain collection

ISSN: 2731-3654