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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant

Fig. 2

Investigation of potential P. oxalicum mutants isolated from each round of mutagenesis. a Clear zones on the two-layer agar gel plates after 8 days. Red circle indicates the isolated colony used for the next round of mutagenesis. b Diameter ratio between clear zone and colony. c RSDE activity. d RSDE activity of the strain A2-13 sub-cultured six times. RSDE activity was determined using RNCF as the substrate. ** (p ≤ 0.01) indicates significant difference between the isolates and the starting strain OXPoxGA15A by Student’s t test. RSDE raw starch-degrading enzyme, RNCF natural raw cassava flour

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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