Fig. 1

Photostability of WSCP–Chl a and free Chl a. Fluorescence measured every 10 min (F) is divided by starting fluorescence (F₀) to show the loss over time. Blue lines represent WSCP–Chl a complexes (2.6 µM) and green lines represent free Chl a (2.6 µM) in 0.03% ß-DM. Each time point is the average of three independent experiments shown with standard error of the mean (SEM). All curves were fitted using an exponential one phase decay model (R² > 0.97). a Comparing the effect of LPMO assay components on the stability of WSCP–Chl a and free Chl a. Assay components were in the following concentrations: Asc (1 mM), TtAA9 (0.035 mg mL⁻¹), 50 mM sodium phosphate buffer (pH 6.3). Conditions: 25 °C and 500 µmol m⁻² s⁻¹ white light (4000 K). b WSCP–Chl a and Chl a under different light and temperature conditions (25 °C/50 °C; 50/200 µmol m⁻² s⁻¹) containing pigments (2.6 µM) and 50 mM sodium phosphate buffer (pH 6.3). c Photostability of WSCP–Chl a and Chl a at different pH. Assay composed of pigments (2.6 µM) and 50 mM potassium phosphate buffer (pH 5-8). Conditions: 25 °C and 500 µmol m⁻² s⁻¹. d 24-h photostability assay performed at 50 µmol m⁻² s⁻¹ with pigments (2.6 µM) and 50 mM sodium phosphate buffer (pH 6.3). All assays were performed with cool white LEDs (4000 K) spectrum