Fig. 2
Light dependent H2O2 formation of WSCP–Chl a under different conditions. Using an Ampliflu™ assay, H2O2 accumulation was measured under different conditions. Blue lines represent WSCP–Chl a complexes and green lines represent unbound Chl a in 0.03% β-DM. Each point is the average of three independent experiments and the corresponding SEM. Light assay components were in the following concentrations: pigments (2.6 µM), and 50 mM sodium phosphate buffer (pH 6.3). a Five different light intensities were tested: 500, 200, 100, 50, and 0 (dark control) µmol m−2 s−1 cool white light (4000 K) spectrum. Assays composed of: pigments (2.6 µM), Asc (1 mM), and 50 mM sodium phosphate buffer (pH 6.3). b Rate constants of H2O2 formation at different light intensities calculated from each curve under the 5 light intensities (0, 50, 100, 200, 500 µmol m−2 s−1) for both WSCP–Chl a and unbound Chl a. c Five different conditions were tested with WSCP–Chl a: 1000, 500, 250, 0 µM Asc and 1000 µM Asc + TtAA9 (0.035 mg mL−1). All samples contained CNF (0.25% w/v). Light intensity was set to 500 µmol m−2s−1. The final H2O2 concentration (30 min) was analyzed with single factor ANOVA for all WSCP–Chl a samples (not including the LPMO control). d Light/dark accumulation of H2O2 with WSCP–Chl a in the presence and absence of Asc, alternating between 5 min in the 50 µmol m−2 s−1 followed by 5 min of fluorescence measurements in the dark