Fig. 3

Gluconic acid determination after light driven TtAA9 time course assays under various Asc feed rates. All three assays were performed at 100 µmol m⁻² s⁻¹ cool white LED (4000 K) spectrum at 25 °C in 50 mM sodium phosphate buffer (pH 6.3), with CNF (0.25% w/v) and either TtAA9 and WSCP–Chl a, TtAA9 and Chl a, TtAA9 without pigment, or WSCP–Chl a as a no enzyme control. Asc feed rates were: a 2 mM Asc, b 1 mM Asc/hour, or c 500 µM every 20 min. Curves are averages of three independent experiments and the SEM of these experiments is shown. Single factor ANOVA was done on the final gluconic acid concentration (120 min) between TtAA9 w/ and w/o WSCP–Chl a. Dark and control reactions can be found in Additional file 1: Fig. S6