Fig. 4

The effect of the single mutation sites on FAP tolerance. a The mutation sites of the mutants BY4742/I12, BY4742/I24 and BY4742/I37. b Exhibition of mutants BY4742/I12, BY4742/I24 and BY4742/I37 mutation sites in an IrrE modeled structure from D. radiodurans. IrrE domains: N-terminal domain colored in cyan; HTH domain colored in green; C-terminal domain colored in pink. c The growth phenotypes on SC-Ura plates of S. cerevisiae strain BY4742 expressing IrrE variants. The strains were cultivated on SC-Ura plates supplemented without or with 0.8 g/L furfural, 3.0 g/L acetic acid, and 0.3 g/L phenol. The experiments were repeated twice