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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: A thermostable bacterial lytic polysaccharide monooxygenase with high operational stability in a wide temperature range

Fig. 5

Probing early inactivation of mgLPMO10 and ScLPMO10C. A “mother reaction” containing 10 g/L Avicel in 50 mM sodium phosphate pH 6.0, 1 µM mgLPMO10 (light grey) or ScLPMO10C (dark grey) and 1 mM AscA was incubated at 50 °C until product formation was stable (the left two sets of bars show product formation in the “mother reaction” after 2 and 4 h). The “mother reaction” was subsequently split into four new reactions to which fresh reactants were added, as indicated in the figure, to the same final concentration as in the starting “mother reaction”. The reactions were then incubated for another 20 h before separating the soluble fraction from the insoluble particles. The solubilized products were degraded to oxidized dimers and trimers by incubation with 1 µM mgCel6A prior to product quantification. All reactions were carried out in triplicates, and error bars show ± SD (n = 3), except for the reaction marked with a star where n = 2

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