Fig. 6

Effect of mgLPMO10 on saccharification of Avicel by Celluclast®. Reaction mixtures were incubated at 50 °C and contained 50 g/Avicel in 50 mM sodium phosphate buffer pH 6.0. The total protein dosage was 4 mg/g glucan, with 100% of Celluclast® (i.e., a mixture of Celluclast® and β-glucosidase in a ratio of 10:1), or Celluclast® and mgLPMO10 in different ratios. Samples from the reaction with mgLPMO10 alone were subjected to postdigestion with a mixture of Celluclast® and β-glucosidase prior to product analysis. BSA was used as control. The numbers over the 48 h bars show the total glucan conversion (% of maximum). All reactions contained 1 mM AscA and were run in triplicates with standard deviations indicated in the figure