A thermostable bacterial lytic polysaccharide monooxygenase with high operational stability in a wide temperature range

Tuveng, Tina Rise; Jensen, Marianne Slang; Fredriksen, Lasse; Vaaje-Kolstad, Gustav; Eijsink, Vincent G. H.; Forsberg, Zarah

doi:10.1186/s13068-020-01834-5

Biotechnology for Biofuels and Bioproducts

Table 1 Reported T_m(app) values for AA9 and AA10 LPMOs in the literature

From: A thermostable bacterial lytic polysaccharide monooxygenase with high operational stability in a wide temperature range

Enzyme (and additives)^a	T_m(app) (°C)	pH (buffer)	Method	Domains	Reversible/irreversible folding	References
mgLPMO10-holo	83.0	6.0 (phosphate)	DSC	AA10-CBM2	Irreversible	This study
mgLPMO10-apo	73.0	6.0 (phosphate)	DSC	AA10-CBM2	Irreversible	This study
mgLPMO10^CD-holo	76.0	6.0 (phosphate)	DSC	AA10	Irreversible	This study
ScLPMO10C-holo	64.1	6.0 (phosphate)	DSF	AA10-CBM2	ND	Jensen et al. [38]
SmLPMO10A-holo	71.2	6.0 (phosphate)	DSF	AA10	ND	Jensen et al. [38]
SmLPMO10A-holo	74.4	5.0 (acetate)	DSC	AA10	Reversible	Sugimoto et al. [45]
SmLPMO10A-apo	65.6	5.0 (acetate)	DSC	AA10	Reversible	Sugimoto et al. [45]
SmLPMO10A	70.3	8.0 (Tris–HCl)	ITF	AA10	ND	Vaaje-Kolstad et al. [46]
EfLPMO10A	72.0	8.0 (Tris–HCl)	ITF	AA10	ND	Vaaje-Kolstad et al. [46]
BaLPMO10A-holo	65.0	5.0 (acetate)	DSF	AA10	ND	Hemsworth et al. [47]
BaLPMO10A-apo	46.0	5.0 (acetate)	DSF	AA10	ND	Hemsworth et al. [47]
BaLPMO10A + EDTA	48.0	5.0 (acetate)	DSF	AA10	ND	Hemsworth et al. [47]
BaLPMO10A-Zn²⁺	52.0	5.0 (acetate)	DSF	AA10	ND	Hemsworth et al. [47]
BaLPMO10A-Ni²⁺	53.0	5.0 (acetate)	DSF	AA10	ND	Hemsworth et al. [47]
BaLPMO10A-Mn²⁺	46.0	5.0 (acetate)	DSF	AA10	ND	Hemsworth et al. [47]
TaLPMO9A-holo	74.3	7.0 (MOPS)	ITF	AA9	Reversible	Singh et al. [48]
TaLPMO9A-apo	65.2	7.0 (MOPS)	ITF	AA9	Reversible	Singh et al. [48]
TaLPMO9A-holo (deglycosylated)	67.9	7.0 (phosphate)	ITF	AA9	Reversible	Singh et al. [48]
MtLPMO9B	75.0^b	7.0 (phosphate)	CD	AA9-CBM1	Irreversible	Frommhagen et al. [49]
MtLPMO9D	68.0^b	7.0 (phosphate)	CD	AA9	Irreversible	Frommhagen et al. [49]
NcLPMO9C-holo	61.5	6.0 (phosphate)	DSF	AA9-CBM1	ND	Kracher et al. [44]^c
NcLPMO9C-holo	44.0	4.0 (acetate)	DSF	AA9-CBM1	ND	Kracher et al. [44]
NcLPMO9C-holo	34.7	4.0 (citrate)	DSF	AA9-CBM1	ND	Kracher et al. [44]
NcLPMO9C-apo (+ EDTA)	52.7	6.0 (phosphate)	DSF	AA9-CBM1	ND	Kracher et al. [44]
NcLPMO9C-holo + AscA	48.8	6.0 (phosphate)	DSF	AA9-CBM1	ND	Kracher et al. [44]
NcLPMO9C-holo + xyloglucan	61.4	6.0 (phosphate)	DSF	AA9-CBM1	ND	Kracher et al. [44]
NcLPMO9C-holo + xyloglucan + AscA	60.4	6.0 (phosphate)	DSF	AA9-CBM1	ND	Kracher et al. [44]
NcLPMO9C	63.0	6.0 (phosphate)	DSC	AA9-CBM1	ND	Kittl et al. [30]
NcLPMO9J	66.9	6.0 (phosphate)	DSC	AA9-CBM1	ND	Kittl et al. [30]
NcLPMO9F	68.9	6.0 (phosphate)	DSC	AA9	ND	Kittl et al. [30]
NcLPMO9E	67.9	6.0 (phosphate)	DSC	AA9-CBM1	ND	Kittl et al. [30]
PvLPMO9A-holo	59.6	7.0 (Polybuffer 96, GE Healthcare)	DSC	AA9-C-term^d	ND	Semenova et al. [50]
PvLPMO9A-apo	49.9	7.0 (Polybuffer 96, GE Healthcare)	DSC	AA9-C-term^d	ND	Semenova et al. [50]

Ligands or other molecules present during the determination of the apparent melting temperature are provided with the enzyme names
ND not detected, DSC differential scanning calorimetry, DSF differential scanning fluorimetry, ITF intrinsic Trp fluorescence, CD circular dichroism
^aThe term “holo” refers to the enzyme with copper bound, while “apo” refers to the metal free enzyme; the presence of other metals and/or substrates is indicated. If nothing is written by the enzyme name, the experiment was performed with unknown copper content
^bTemperatures estimated based on CD diagrams in Frommhagen et al. [49]
^cThe table only shows a selection of stability data presented in the study by Kracher et al. [44]
^dC-terminal peptide of unknown function

Back to article page

ISSN: 2731-3654

Contact us

General enquiries: journalsubmissions@springernature.com