Fig. 3

Activity of TausLPMO9B in the presence of various reductants. The graph shows the release of oxidized products by TausLPMO9B in reactions with different reductants. The chemical structures of the reductants are indicated above the bars. Reactions contained 1 µM LPMO, 0.1% (w/v) PASC and either 1 mM of small reducing compounds, 1 mg/mL of kraft lignin or 1% (v/v) of water-soluble lignin (ws-lignin). Product release was measured after 24-h incubation at 45 °C and pH 5.0 and is reported as the sum of the area of peaks corresponding to C1-oxidized cello-oligosaccharides (DP2–DP6). Due to the high background signal of the extracted lignin (ws-lignin) overlapping with signals of the LPMO products, the results for this set of experiments were background corrected. In the control sample, PASC was incubated with ws-lignin but without enzymes, using the same reaction conditions as in the reaction containing the enzyme. Reactions were run in triplicate, error bars indicate standard deviation