Fig. 5

Effect of H₂O₂ and ascorbic acid on TausLPMO9B activity on cellulose. 0.1% (w/v) PASC was incubated with 1-µM TausLPMO9B acid in sodium acetate buffer pH 5.0 at 45 °C with different combinations of reductant/H₂O₂. a, b: 1-mM ascorbic acid and supplementation of different concentrations of H₂O₂ (0-200 µM). At time ‘0’, the reactions were supplemented with 0–200 µM H₂O₂. Product formation was assessed based on the sum of peak areas corresponding to C1-oxidized cello-oligosaccharides in the HPAEC–PAD chromatograms. c, d 0.1% (w/v) PASC was incubated with 1 µM TausLPMO9B and either 100-µM or 1-mM ascorbic acid in sodium acetate buffer pH 5.0 at 45 °C. At time ‘0’, the reactions were supplemented with 0 (ultra pure water) or 25-µM H₂O₂. Product formation was assessed based on the sum of peak areas corresponding to C1-oxidized cello-oligosaccharides in the HPAEC–PAD chromatograms. a, c Product accumulation in the first 120 min, the lines connecting the points are drawn for illustration purposes only; b, d final product levels after 24 h. The error bars indicate standard deviations of three replicates