Fig. 4

Contribution of lignin-derived compounds in the AIR fraction of Saccharum spp. hybrids. Genotype Q208 is demarcated in dark blue, and KQ228 is demarcated in tan. The compounds originate from guaiacyl (G) (a–e, h–i), syringyl (S) (g, j) and hydroxycinnamic acids (HC) (k, l). Note: hydroxycinnamic acids were detected separately via HPLC determination, and are presented as a % of alcohol-insoluble residue (AIR). Guaiacyl and syringyl monolignols presented as total % of S and G lignin. Abbreviations, TI: top internode; MI: middle internode; BI: bottom internode; first visible dewlap leaf: L1; fifth visible dewlap leaf: L5; R: root. Letters above each bar indicate the presence of a significant difference between values within the same genotype. Error bars ± 1 S.D. from biological triplicates. Significance was calculated via one-way ANOVA, with the post hoc LSD test to separate statistically dissimilar groups. Statistical analysis was measured separately within each genotype