Fig. 4

Simulations of impact of C/N ratio on lipid production by C. oleaginosus ATCC 20509. a: Simulation using the surface response model [25], b: A comparison of lipid production at a fixed N concentration at 0.3 (g/l) between surface response method in [25] and iNP636_Coleaginosus_ATCC20509 using a standard biomass (static) and condition-specific (dynamic) biomass. c: iNP636_Coleaginosus_ATCC20509 simulation using the proposed condition-specific biomass objective function, d: iNP636_Coleaginosus_ATCC20509 simulation using a standard biomass objective function. Glucose is used as a carbon source as was used in the response method in [25]