Fig. 8

In silico flux changes in C. oleaginosus ATCC 20509 at different C/N ratio with a 16 g carbon; b 24 g carbon; c 32 g carbon using glycerol as a sole carbon source. A C/N ratio of 6 g/g was used as reference point to calculate Z-score for each C/N ratio. The C/N (g/g) refers to the ratio between the uptake rates of carbon and nitrogen source. Z-score > 0 indicates an increase in flux compare to that at reference point; Z-score < 0 indicates a decrease in flux compared to that at reference point. PPP–pentose phosphate pathway; AAs metabolism–amino acids metabolism. Each dot in the graph represents a reaction in the corresponding pathway