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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: LACCASE14 is required for the deposition of guaiacyl lignin and affects cell wall digestibility in poplar

Fig. 5

Enzymatic oxidation of monolignols by recombinant PtoLAC14 protein. a Quantification of PtoLAC14 activity in E. coli, with ABTS as the substrate. b The purified recombinant PtoLAC14 protein analyzed by SDS-PAGE. c The activity of purified recombinant PtoLAC14 protein, with coniferyl alcohol and sinapyl alcohol as the substrate. d Kinetic properties of PtoLAC14 in vitro. Km and Vmax were determined by linear regression of v against v/s (Eadieā€“Hofstee plot) with at least five data points. The data are mean values from three independent measurements

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