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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Identification of a cytosine methyltransferase that improves transformation efficiency in Methylomonas sp. DH-1

Fig. 4

The transformation efficiency of methylated plasmid DNAs. a The overall biosynthetic pathway towards carotenoid. The psy gene is indicated. b Transformation efficiencies of non-methylated plasmids (light gray bar) and methylated plasmids (dark gray bar) in Methylomonas sp. DH-1. The maps of the two plasmids are shown in Fig. 3a. Standard deviations were calculated from triplicates. The asterisk (*) denotes p values < 0.05. c Carotenoid intensity in Methylomonas sp. DH-1 cells after transformation with the methylated psy plasmid. The intensity was measured using multi-detection microplate reader, and the carotenoid intensity was obtained 8 h after cultivation. Standard deviations were calculated from triplicates

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