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Table 1 Summary of bio-based production of d-glucaric acid.

From: Cell-based and cell-free biocatalysis for the production of d-glucaric acid

 

Year

Hosts

Genes

Carbon sources

Titer (g/L)

Yield (g/g)

Refs.

In vivo

2009

E. coli BL21 Star (DE3)

ino1, MIOX, udh

Glucose

1.13

0.113

[18]

2010

E. coli BL21 Star(DE3)

ino1, MIOX, udh

Glucose

2.50

0.250

[22]

2014

E. coli MG1655

ino1, SUMO-MIOX, udh

Myo-inositol

4.85

0.449

[26]

2015

E. coli IB1486

ino1, SUMO-MIOX, udh

Glucose

1.56

0.124

[44]

2017

E. coli L19S

ino1, MIOX, udh

Glucose

> 0.8

–

[45]

2018

E. coli K-12

ino1, MIOX, udh

Glucose

1.98

0.198

[48]

2018

E. coli BL21 Star(DE3)

cscB, cscA, cscK, ino1, MIOX, udh, suhB

Sucrose

1.42

0.27

[19]

2020

E. coli BL21 Star(DE3)

ino1, MIOX, udh, suhB

Glucose

5.35

0.467

[40]

2016

S. cerevisiae CEN.PK2-1D opi1Δ

ino1, inm, MIOX, udh

Glucose

0.98

0.33

[50]

2016

S. cerevisiae CEN.PK2-1D opi1Δ

ino1, inm, MIOX, udh

Glucose, myo-inositol

1.6

–

[50]

2016

P. pastoris GS115

MIOX, udh

Glucose, myo-inositol

6.61

–

[50]

2018

S. cerevisiae BY4741 opi1∆

ino1, MIOX, udh

Glucose, myo-inositol

6.0

–

[55]

2020

S. cerevisiae

ino1, inm, MIOX, udh, vhB

Glucose

6.38

0.128

[58]

In vitro

2016

Soluble enzymes

xyn, AG, udh

Hemicellulose

–

–

[67]

2019

Soluble enzymes

SP, PGM1, MIPS, IMPASE, MIOX, udh, nox

Sucrose

7.31

0.75

[64]

2020

Soluble enzymes

AxyAgu115A, GOOX

Xylan

–

–

[68]

2020

Immobilized enzymes

PGM1, IPS, IMP, MIOX, udh, nox

Glucose-1-phosphate

1.70 ± 0.04

0.202

[69]