Fig. 4From: Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteinsa The performance of the strain engineered with the TCA cycle. The experiment was carried out using crude glycerol. Typical profiles were shown for N31-5AK(HDT) strain with cell density (black circle), glycerol consumption (white circle), and the volumetric activity of HDT (solid bar). b The SDS-PAGE analysis of soluble proteins from recombinant strains in crude glycerol. At the end of the experiment, bacterial culture of 1 mL were harvested and processed for the analysis of SDS-PAGE as described. The arrow indicated the position of soluble HDT. Keys: lane 1, protein marker; lane 2, proteins of BAD-5/pET-TrChHDT strain; lane 3, proteins of N31(HDT) strain; lane 4, proteins of N31-5AK(HDT) strainBack to article page