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Table 1 Summary of the production kinetics for strains with engineered traits

From: Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins

Strain S OD (T0.5) HDT Y Manipulated gene
arcA zwf pgl gldA dhaKLM gltA glpF
Pure glycerol
 BAD-5 0.04 ± 0.00 0.28 ± 0.01 (1.54) 0.09 ± 0.01 225
 N31 0.13 ± 0.01 0.43 ± 0.02 (1.35) 0.19 ± 0.01 216 + + +
 N31(HDT) 0.13 ± 0.01 0.53 ± 0.02 (1.32) 0.22 ± 0.01 244 + + +
 N31-5(HDT) 0.32 ± 0.02 1.55 ± 0.05 (0.91) 1.68 ± 0.13 672 + + + + +
 N31-Arc(HDT) 0.37 ± 0.02 1.61 ± 0.06 (0.91) 2.59 ± 0.19 948 + + + + +
 N31-5AK(HDT) 0.40 ± 0.03 1.71 ± 0.08 (0.84) 2.83 ± 0.22 969 + + + + + +
Crude glycerol            
 N31-5AK(HDT) 0.40 ± 0.03 1.76 ± 0.09 (0.84) 2.84 ± 0.22 969        
  1. Note that BAD-5 and N31 strains harbored plasmid pET-TrChHDT for expression of HDT. Glycerol consumption rate (∆S) in g/L h, final biomass (OD) in g dry cell weight (DCW)/L, the protein production (HDT) in U/mL, and the protein yield based on glycerol (Y) in U/g were calculated based on experimental data. Numbers in parentheses indicate the doubling time (T0.5) in h of strains
  2. Symbols for manipulated genes: +, enhancement; −, wild type, , deletion