Table 1 Summary of the production kinetics for strains with engineered traits
From: Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
Strain | â–³S | OD (T0.5) | HDT | Y | Manipulated gene | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
arcA | zwf | pgl | gldA | dhaKLM | gltA | glpF | |||||
Pure glycerol | |||||||||||
BAD-5 | 0.04 ± 0.00 | 0.28 ± 0.01 (1.54) | 0.09 ± 0.01 | 225 | − | − | − | − | − | − | − |
N31 | 0.13 ± 0.01 | 0.43 ± 0.02 (1.35) | 0.19 ± 0.01 | 216 | − | − | − | + | + | − | + |
N31(HDT) | 0.13 ± 0.01 | 0.53 ± 0.02 (1.32) | 0.22 ± 0.01 | 244 | − | − | − | + | + | − | + |
N31-5(HDT) | 0.32 ± 0.02 | 1.55 ± 0.05 (0.91) | 1.68 ± 0.13 | 672 | – | + | + | + | + | − | + |
N31-Arc(HDT) | 0.37 ± 0.02 | 1.61 ± 0.06 (0.91) | 2.59 ± 0.19 | 948 | △ | + | + | + | + | − | + |
N31-5AK(HDT) | 0.40 ± 0.03 | 1.71 ± 0.08 (0.84) | 2.83 ± 0.22 | 969 | – | + | + | + | + | + | + |
Crude glycerol | |||||||||||
N31-5AK(HDT) | 0.40 ± 0.03 | 1.76 ± 0.09 (0.84) | 2.84 ± 0.22 | 969 | |||||||