Table 1 Efficiency of RNP-based CRISPR–Cas9 system for genetic modifications
Genetic modification | Total number of transformants | Number of correct transformants | Efficiency (%) | Corresponding engineered strains |
|---|---|---|---|---|
Δgox | 8 | 3 | 37.5 | SAP-1 |
Δoah | 16 | 2 | 12.5 | SAP-1 |
ΔAnDct::GPDAp-AcDCT-tTrpC | 24 | 2 | 8.3 | SAP-2 |
Δoah::GPDAp-frd-tTrpC | 11 | 2 | 18 | SAP-3 |