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Table 1 Efficiency of RNP-based CRISPR–Cas9 system for genetic modifications

From: Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR–Cas9 system for succinic acid production from renewable biomass

Genetic modification Total number of transformants Number of correct transformants Efficiency (%) Corresponding engineered strains
Δgox 8 3 37.5 SAP-1
Δoah 16 2 12.5 SAP-1
ΔAnDct::GPDAp-AcDCT-tTrpC 24 2 8.3 SAP-2
Δoah::GPDAp-frd-tTrpC 11 2 18 SAP-3