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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuels

Fig. 2

Growth restoration of the rnf mutant on H2 + CO2 after complementation with the plasmid pMTL_8312_Ptet_SrnfH. The A. woodii wild type (■), the wild type containing the vector pMTL8312_Ptet without the rnf operon (♦), the rnf mutant (▲), and the complemented strain Δrnf pMTL_8312_Ptet_SrnfH () were grown in complex medium under a H+ CO2 [80:20 v/v] atmosphere with a pressure of 1.0 × 105 Pa. Plasmid-containing strains were induced with 400 ng/ml atet at an OD600 of 0.8–1.1. The OD600 was followed over 95 h. N = 2 independent experiments

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