Fig. 3From: Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuelsPurification of the overproduced Rnf complex from A. woodii in A. woodii Δrnf. A. woodii Δrnf + pMTL8312_Ptet_rnf was grown at 30 °C in complex medium. After reaching an OD600 of ~ 0.2–0.3, Rnf production was induced with 400 nM aTet. Cells were harvested, crude extract was prepared, and membranes were isolated by ultracentrifugation. After solubilization with DDM, proteins were purified using a Ni2+-affinity column. Elution fractions E2 and E3 were pooled and proteins (20 µg) were separated on an SDS-PAGE and either stained with Coomassie Brilliant Blue or silver. M, molecular mass standardBack to article page