Fig. 4From: Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuelsWestern Blot analysis of Rnf subunits and detection of FMN in Rnf subunits of the purified Rnf complex from A. woodii. Purified proteins (20 µg) were separated on an SDS-PAGE and were either transferred onto nitrocellulose membranes and blotted against His6, RnfC, RnfB, and RnfG antibodies (a) or were placed under UV light (280 nm) for visualization of flavin-containing subunits (b)Back to article page