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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuels

Fig. 5

Size determination of the purified Rnf complex of A. woodii. After purification via a Ni2+ column, 10 µg of the purified Rnf complex were loaded on a native polyacrylamide gel and stained with Coomassie Brilliant Blue (a) and 340 µg of the complex were loaded onto a Superose 6 column (b), which was eluted in buffer-containing 50 mM Tris–HCl, pH 8.0, 20 mM MgSO4, 5 µM FMN, 0.02% DDM, 2 mM DTE, and 4 µM resazurin, respectively

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